Determination of dexmedetomidine in human plasma by solid phase extraction with gas chromatography-mass spectrometry
10.03618/j.issn.1001-5728.2017.05.014
- VernacularTitle:固相萃取-气相色谱-质谱法检验人血浆中的右美托咪定
- Author:
Ruihua WANG
1
;
Rui LIU
;
Yujing LUAN
;
Ying DONG
;
Xinxin REN
Author Information
1. 公安部物证鉴定中心
- Keywords:
forensic toxicological analysos;
dexmedetomidine;
plasm;
solid phase extraction;
gas chromatography-mass spectrometry
- From:
Chinese Journal of Forensic Medicine
2017;32(5):497-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the analytical method of dexmedetomidine in human plasma by solid phase extraction with gas chromatography-mass spectrometry(SPE-GC/ MS). Methods The human plasma were extracted with a solid phase extraction(SPE) and determined by GC/MS. Results The lowest detectable limit was 0.05μg/mL, the standard curve was linear over the range of 0.2μg/mL~5μg/mL. The absolute recovery was 86.1%~91.5%. The intra-and interday precision was within 7.86% at three concentrations. Conclusion Since the procedure proved to be simple, quick and sensitive, it could be used for the determination of dexmedetomidine in human plasma.
