The research on the technology of vacuum coating developing fingerprints and the profiling of DNA on the objects of cloth
10.03618/j.issn.1001-5728.2017.05.015
- VernacularTitle:布料上掌纹的真空镀膜显现及DNA检验的研究
- Author:
Shuai SUN
1
;
Zishu JIA
;
Qingxia ZHANG
;
Yulong HU
;
Luyan XUE
;
Jinjie LIU
;
Li LIU
;
Hui TANG
Author Information
1. 山西医科大学晋祠学院
- Keywords:
forensic biological evidence;
latent palmprints;
loci numbers;
vacuum coating;
pigments;
cloth
- From:
Chinese Journal of Forensic Medicine
2017;32(5):500-503
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To conduct a research on the possibility and effect factors of latent fingerprints development in clothing objects after vacuum coating, and extracting fingerprints DNA and to probe in the relation among DNA template quantity and genetic loci numbers tested, and the rfu value after coating. Methods To select two groups that are free sweat hands and sweat hands and have them press their fingerprints on the cloth, after coating, and to analyze the effect of time, to quantify and test the targeted fingerprints DNA, to compare the locus numbers tested between white and black cloth. Results As the time is prolonged, the locus numbers tested decrease. The locus numbers tested on the group of sweat hands using the same method after the same placed time are lager than the free sweat hands. When the value of rfu is 600 above, the ratio of the locus numbers tested is more than 90% and the threshold of templates is 0.013ng. The locus numbers tested of white cloth is larger, comparing with black cloth when using the same method. What is more, there exists an prohibitive influence of pigments of the dyed cloth over the PCR amplification, to put it further, the loci numbers tested will be trimmed. Conclusion The technology of vacuum coating can be well used in the area of detecting fingerprint DNA.