Analysis for genetic polymorphism of OSU49 locus on Y chromosome
10.03618/j.issn.1001-5728.2017.05.016
- VernacularTitle:Y染色体OSU49基因座遗传多态性分析
- Author:
Xi LI
1
;
Juan MENG
;
Yanmei HUANG
;
Yanqin XU
;
Liwei GUO
;
Qian ZHANG
Author Information
1. 新乡医学院法医学院法医物证学教研室
- Keywords:
forensic biological evidence;
complex repeats;
STR;
Y-STR locus;
genetic polymorphism
- From:
Chinese Journal of Forensic Medicine
2017;32(5):504-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate genetic polymorphism of OSU49 locus on Y chromosome among 300 unrelated males in Henan Han population and to assess its value in forensic science. Methods Under the case of informed consent, the blood samples were collected from 300 male individuals in Henan Han. The primer was labeled with florescent. PCR products were separated from ABI 3130 genetic analyzer. According to the typing test results, the sequence of different alleles was analyzed. Result OSU49 locus contained both pentanucleotide and tetranucleotide core sequence. In the Han population of Henan, the motif of OSU49 locus was showed (CTTTC)pCTT(CCCT)7 T(CTTTC)1(TCTT)5(TCCT)m(TCTT)nTCT(TCCT)4. The number of pentanucleotide repeats varied from12 to 17, and the number of tetranucleotide repeats varied from 20 to 30. Nomenclature for allele was according to the length of the fragment. A total of 34 alleles were detected. The gene diversity was 0.9186 and the discrimination power was 0.9155. Conclusion The complex repeats of locus OSU49 was highly polymorphic in Henan Han population, which can be used in forensic science and human genetics studies.
