Rapid detection of oleandrin and adynerin in human blood by HPLC-MS/MS

VernacularTitle:HPLC-MS/MS法快速检测人体血液中的夹竹桃毒素
Author: Rui SONG ¹ ; Jifeng WU ; Haiyan LIU ; Yujing LUAN
Author Information

1. 济南市公安局刑事科学技术研究所
Keywords: forensic toxicological analysis; HPLC-MS/MS; human blood; oleandrin; adynerin
From: Chinese Journal of Forensic Medicine 2017;32(5):514-517
CountryChina
Language:Chinese
Abstract: Objective To establish the analytical method of oleandrin and adynerin in human blood by HPLC-MS/MS. Methods After protein sediment by acetonitril, the concentrations of oleandrin and adynerin in human blood were quantitatively determined by HPLC-MS/MS. The qualitative analysis was conducted based on retention time and MRM ions. Besides, the standard curve method was used for quantification. Results The detection limits of both oleandrin and adynerin were 0.5ng/mL, the linear range was from 1ng/mL to 1mg/mL, with a recovery rate of 75.2%~95.7%. Conclusion The detecting protocol has the advantages of high sensitivity, fast and high accuracy with a relatively wide linear range, which is especially suitable for rapid detection of oleander toxins, alexandrine and adynerin in particular, in human blood in poisoning cases.