Mutation analysis of autosomal and X chromosomal STR in gynecologic and breast cancer
10.13618/j.issn.1001-5728.2017.04.003
- VernacularTitle:妇科肿瘤和乳腺癌组织常染色体和X染色体STR的突变分析
- Author:
Lijuan SUN
1
;
Shujin LI
;
Guangping FU
;
Mei BAI
;
Chunnan DONG
;
Bo YANG
;
Pingren CHEN
;
Zhandong WANG
;
Lihong FU
;
Bin CONG
Author Information
1. 河北医科大学法医学院
- Keywords:
forensic biological evidence;
gynecologic cancer tissue;
breast cancer tissue;
STR mutation;
microdissection
- From:
Chinese Journal of Forensic Medicine
2017;32(4):350-353,358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mutation types and disciplines of STR commonly used in forensic in gynecologic and breast cancerand investigate the application of microdissection in forensic practice involving tumor tissue. Methods DNA of tumor tissues, adjacent normal tissues and peripheral blood from 62 patients with breast cancer, 62 patients with gynecologic cancer and 10 patients with benign gynecologic tumor were amplified by PowerPlex 21 System kit and Argus X-12 kit. Capillary electrophoresis of PCR products was carried out on an ABI 3130 Genetic Analyzer to obtain genotypes. Some tumor tissues with STR variation were microdissected. Results The genotype of peripheral blood in cancer patient was consistent with that of corresponding normal tissue. 4 types of STR variations were found in 46.77% gynecologic cancer tissues, compared with that in benign tumor tissues and breast cancer, the difference of STR variation was significant(P<0.01,P=0.009). The genotype of stromal cells separated by microdissection was consistent with that of corresponding adjacent normal tissue. Conclusion The STR loci detected in the study with poor stability are not suitable for forensic cases involving gynecologic cancer tissues. The genotype of stromal cells separated accurately from tumor tissues by microdissection could represent the normal DNA genotype of the individual with cancer. Microdissection is an effective solution in forensic cases with tumor tissues.
