Comparative study of PCR effect of rapid PCR instrument and common PCR instrument
10.13618/j.issn.1001-5728.2017.04.012
- VernacularTitle:快速PCR仪与普通PCR仪扩增效果的比较研究
- Author:
Huajie BA
1
;
Aihua ZHU
;
Yanan LIU
;
Ziqing LIN
Author Information
1. 常州市公安局物证鉴定所
- Keywords:
forensic biological evidence;
9947A standard;
Rapid PCR instrument;
Common PCR instrument
- From:
Chinese Journal of Forensic Medicine
2017;32(4):382-384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the PCR effect of rapid PCR instrument and common PCR instrument. Methods The concentration of 9947A standard was diluted to 0.1, 0.05, 0.025, 0.0125, 0.0063 ng/μL, 100 samples from each concentration group to establish PCR reaction system with Identifiler? Plus PCR Amplification Kit, 50 samples of them test with rapid PCR instrument (Speed cycler2 thermocycler), the other 50 samples test with common PCR instrument (9700 thermocycler). Detection of PCR product with 3500xL Genetic Analyser, the STR typing of both groups of each concentration group should be compared. Results The success rate of both thermocyclers have no significant difference (P>0.05); The success number of STR typing of common PCR instrument (13.7±1.0; 11.3±1.5) were higher than rapid PCR instrument (13.1±1.3; 9.9±1.9) when the concentration of 9947A were 0.0125, 0.0063ng/μL (P=0.029; P<0.001); The peak height of DNA typing map obtained from common PCR instrument (18931±4625;13437±3165; 5752±1344) were higher than rapid PCR instrument (16929±4034; 11815±4120; 4865±1401) when the concentration of 9947A were 0.1, 0.05, 0.025ng/μL (P=0.023; P=0.030; P=0.002). Conclusions The rapid PCR instrument could achieve the equal success rate to the common PCR instrument with less time, which revealed that the rapid PCR instrument was suitable for application in practical cases; The quality of STR typing from common PCR instrument may be more higher.
