A new methodology of DNA polymorphism detection from standard long gun
10.13618/j.issn.1001-5728.2017.04.013
- VernacularTitle:建立检测制式长枪上DNA多态性的新方法
- Author:
Xiuqiao YANG
1
;
Lin MIAO
;
Hongliang XIE
;
Lei HUANG
;
Shida SU
;
Shunyong PAN
;
Faming ZENG
;
Dian ZHAI
;
Baowen CHENG
Author Information
1. 昆明医科大学法医学院
- Keywords:
forensic biological evidence;
direct PCR;
silicon membrane method;
standard long gun;
genotype
- From:
Chinese Journal of Forensic Medicine
2017;32(4):385-387,392
- CountryChina
- Language:Chinese
-
Abstract:
Objective A new methodology was established to efficiently obtain the genotype of DNA remained on standard long gun. Methods Direct PCR and silicon membrane method were combined to detect DNA polymorphism of a total of 240 samples at 5 different positions from 48 standard long guns. Results Combining direct PCR and silicon membrane method, we obtained full DNA profiles in 42 out of 48 standard long guns, with a detection rate up to 87.50%. Conclusion The results demonstrate that the combination of direct PCR and silicon membrane method provide a quick and accurate way to detect DNA polymorphism on the standard long gun.
