The method to estimate DNA concentration by "coffee ring" pattern
10.13618/j.issn.1001-5728.2017.04.017
- VernacularTitle:用"咖啡环"法估测DNA含量
- Author:
Yaqin LI
1
;
Guisen ZHAO
;
Fei ZHAN
Author Information
1. 河南科技大学法医学院
- Keywords:
forensic biological evidence;
coffee ring effect;
DNA quantitatve;
ethidium bromide
- From:
Chinese Journal of Forensic Medicine
2017;32(4):400-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of "coffee ring" effect in DNA high-throughput evaluation. Methods we mixed uniformly DNA solution and EB solution, then dripped different size of droplets on 5 kinds of substrates. After evaporation, the coffee ring was observed and photographed with LG2020 gel imaging analysis system. Analyzed the correlation among ring formation conditions,ring fluorescence intensity and DNA concentration, and looked for the sensitivity and applicable range of the method. Result DNA - EB solution can form fluorescent ring after surface evaporation on the slide, transparent polystyrene plate carrier and so on. The DNA detection limit can be up to 2ng/μl. DNA concentration and loop integral optical density fitting Logistic equation(R2=0,999) within 2ng/μl~1000ng/μl. Conclusion The coffee ring method is simple, fast and low equipment requirement,which can be used for high-throughput evaluation of DNA solution.
