Continuously Monitoring of Concentration of Extracellular Ascorbic Acid in Spinal Cord Injury Model
10.11895/j.issn.0253-3820.171091
- VernacularTitle:脊髓损伤模型中胞外抗坏血酸浓度的活体在线分析
- Author:
Yang LYU
1
;
Wen Ya ZHANG
;
Lei TAN
;
Liang Wen JI
;
Ping YU
;
Qun Lan MAO
;
Fang ZHOU
Author Information
1. 北京大学第三医院
- Keywords:
Ascorbic acid;
Spinal cord injury;
In vivo microdialysis;
On-line electrochemical detection
- From:
Chinese Journal of Analytical Chemistry
2017;45(11):1595-1599
- CountryChina
- Language:Chinese
-
Abstract:
Acute traumatic spinal cord injury (SCI) represents one of the most devastating injuries that afflict the human body. Ascorbic acid ( AA) plays an important role in mammalian central nervous system, especially in SCI. In this study, the change of AA concentration after SCI was investigated by using an on-line electrochemical method integrated with in vivo microdialysis. A microdialysis probe (2 mm in length) was implanted into the spinal cord of an anesthetized rat (Thoracic-10). Microdialysis perfusate (2 μL/ min) was collected in the sample loop of an on-line injector for direct injection onto a glassy carbon electrode which was modified with the heat-treated single-walled carbon nanotubes (SWNTs). Normal ascorbic acid concentration in the extracellular fluids of spinal cords was (26. 17 ± 1. 25) μmol/ L (n =8). The experimental spinal cord injury, induced by a lesion at T-10, significantly increased the extracellular ascorbic acid levels to (53. 24± 1. 95) μmol/ L (n =8). This study provides the experimental evidence on the essential roles of ascorbic acid in spinal cord injuries.
