A preliminary study of the effects of Miseq FGx sequencing system for degraded bone sample testing
10.03618/j.issn.1001-5728.2017.05.018
- VernacularTitle:应用Miseq FGx平台检测降解骨骼样本效果初探
- Author:
Youfang ZHANG
1
;
Qin WANG
Author Information
1. 浙江警察学院
- Keywords:
forensic biological;
degraded bone samples;
Miseq FGx
- From:
Chinese Journal of Forensic Medicine
2017;32(5):512-513,517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of Miseq FGx sequencing system for degraded bone sample testing. Methods Ten degraded bone samples that produced unsatisfactory STR typing with PCR-CE were tested using Miseq FGx, so as to compare the detection result and detection rate of the STR loci between the two systems. Results The detection rates of all the STR loci were higher with MiSeq FGx than with PCR-CE; some of the STR loci that might have not been detectable with PCR-CE were probably detectable with Miseq FGx. Sequencing with Miseq FGx also provided abundant information on the genetic markers including X-STR, Y-STR and SNP. Conclusion MiSeq FGx presents high application value for degraded bone sample testing.
