The exploration of cell-free DNA in human humoral biological samples with the magnetic beads direct absorption method

VernacularTitle:磁珠直接吸附法对体态检材游离DNA的提取
Author: Xiaoming HAO ¹ ; Jinguang SUN ; Chunbao GOU ; Zheren FAN ; Anli LIU ; Wujun ZHAO ; Zheng ZHANG
Author Information

1. 重庆市巴南区公安分局刑事科学技术室
Keywords: forensic biological evidence; cell free DNA; magnetic beads; direct adsorption method; biological sample
From: Chinese Journal of Forensic Medicine 2017;32(4):379-381
CountryChina
Language:Chinese
Abstract: Objective The magnetic beads direct adsorption method was used to extract the cell-free DNA (cfDNA) from three kinds of human humoral biological samples, including urine, saliva and blood, as to provide a reference for forensic cfDNA research and forensic inspection. Methods The cfDNA was isolated from humoral samples by centrifuging, and the cfDNA was extracted with the method of magnetic beads direct adsorption. Then the samples were sequentially amplified with Identifiler-Plus amplication kit, and the STR genotyping was detected by ABI 3500 Analyzer. Results The cfDNA was detected from all the three kinds of samples. The detection rate of cfDNA from the blood samples was 100%, the saliva was 90%, and the urine was 70%. Conclusion The results suggest that human humoral biological samples contain cfDNA. What's more, the magnetic beads direct adsorption method can be used to extract cfDNA efficiently and conveniently.