Effect of Guizhi Fuling capsule and its extracts on human breast cancer cells proliferation
10.3867/j.issn.1000-3002.2017.10.098
- Author:
YU ZI-RU
1
;
LI LI
;
WANG JIN-HUA
;
WANG ZHEN-ZHONG
;
XIAO WEI
;
DU GUAN-HUA
Author Information
1. Beijing Key Laboratory of Drug Targets Identification and Drug Screening
- Keywords:
high throughput screening;
Guizhi Fuling capsule;
breast cancer;
MCF-7 cell;
MDA-MB-231 cell;
sulforhodamine B
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(10):1013-1014
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the effect of Guizhi Fuling Capsule active pharmaceutical ingredient (API) and its fractions on human breast cancer cells proliferation by high-throughput screening assay. METHODS The crude fractions were obtained from the extraction and elution of the API of Guizhi Fuling Capsule, and 929 standard fractions were obtained by the optimal separation conditions. Sulforhodamine B (SRB) method was used to evaluate the effects of the Guizhi Fuling capsule API and 929 kinds of fractions on the proliferation of human breast cancer cells MCF- 7 and MDA- MB- 231. RESULTS The Guizhi Fuling capsule API had a strong ability to inhibit the proliferation of MCF-7 cells at high concentration and the ability to inhibit MDA-MB-231 cells' proliferate at low concentration follow?ing 72 h treatment;some samples of 929 fractions (5 μg·mL-1) was found to have a breast cancer cell growth inhibition rate above 50%, without toxicity on HUVECs proliferation. CONCLUSION The API of Guizhi Fuling capsule had significant cytotoxicity effects on these two human breast cancer cells, with significant concentration- and time-dependent manner.
