Effect of regulating peroxisome proliferator-activated receptor γ on soluble endoglin expression in first-trimester trophoblasts
10.3760/cma.j.issn.1007-9408.2017.10.006
- VernacularTitle:调节过氧化物酶体增殖物激活受体γ对早孕期滋养细胞可溶性endoglin表达的影响
- Author:
Yuchun ZHU
1
;
Xiaolan YU
;
Yongbing GUO
;
Yu SUN
;
Huixia YANG
Author Information
1. 100034,北京大学第一医院妇产科
- Keywords:
Pregnancy trimester,first;
Trophoblasts;
PPAR γ;
Antigens,CD;
Receptors,cell surface;
Pre-eclampsia
- From:
Chinese Journal of Perinatal Medicine
2017;20(10):724-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of regulating peroxisome proliferator-activated receptor γγ (PPAR γ) on soluble endoglin (sEng) expression in first-trimester trophoblasts via an in vitro study.Methods Chorionic villus were collected from 20 samples of first-trimester artificial abortion in Peking University First Hospital from July 1 st to 31 st,2016.Primary culture of trophoblast cells was performed.Trophoblast cells from each sample were divided into three groups,which were PPAR γ antagonist group,PPAR γ antagonist and PPAR γ agonist group,and control group.Supematant sEng level was detected in each group by enzyme linked immunosorbent assay (ELISA).Paired-sample t test was used for statistical analysis.Results Compared with the control group,trophoblast cells in the PPAR γ antagonist group grew slower and were reduced in number.No significant difference in growth or morphology of trophoblast cells was observed between the PPAR γγ antagonist and PPAR γγ agonist group and the control group.Supernatant sEng level was elevated in the PPAR γ antagonist group,but was not significantly changed in the PPAR γ antagonist and PPAR γ agonist group as compared with that in the control group [(124.1 23.8) vs (94.0± 12.7) pg/ml,t=-4.31,P＜0.05;(87.1 ± 10.6) vs (94.0± 12.7) pg/ml,t=1.62,P=0.12).Conclusions Suppression of PPAR γ promotes sEng expression in trophoblast cells and that can be reversed by PPAR γ agonist.
