Effects of Smo gene silencing on cell activity and apoptosis of human cer-vical carcinoma HeLa cells
10.3969/j.issn.1000-4718.2017.11.015
- VernacularTitle:沉默Smo基因对人宫颈癌HeLa细胞活力及凋亡的影响
- Author:
Ling SONG
1
;
Qiang ZHOU
;
Na LI
;
Jie YU
;
Yang LI
;
Chi ZHANG
Author Information
1. 商丘医学高等专科学校
- Keywords:
Smo gene;
Cervical carcinoma;
RNA interference;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(11):2015-2019
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Hedgehog(Hh) signaling pathway on the viability and apoptosis of cervical carcinoma cells by shRNA technique to knock down Smoothened (Smo) gene. METHODS:Smo shRNA was used to transfect the cervical carcinoma HeLa cells. The expression of Smo and Gli1 at mRNA and protein levels in the He-La cells was determined by RT-PCR and Western blot,respectively. The effect of Smo gene silencing on the growth of the cells was measured by MTT assay. The apoptosis and cell cycle were determined by flow cytometry. RESULTS:Compared with control group,the mRNA and protein expression of Smo and Gli1 were evenly reduced obviously after transfected with Smo shRNA for 72 h(P<0.05). The viability of HeLa cells transfected with Smo shRNA was significantly inhibited. The percentages of the cells in G0/G1phase and early apoptosis rate were obviously higher in Smo shRNA transfection group than those in control group. CONCLUSION:Smo gene silencing effectively inhibits the cell growth and induces the apop-tosis of human cervical carcinoma cells.
