α2-macroglobulin alleviates X-ray induced obstacle on osteogenic differen-tiation of human bone marrow mesenchymal stem cells
10.3969/j.issn.1000-4718.2017.11.018
- VernacularTitle:α2-巨球蛋白减轻X射线所致人骨髓间充质干细胞成骨分化障碍
- Author:
Yang LIU
1
;
bo Xiang KONG
;
Jie LI
;
ying Xue CHEN
;
yu Chuang WEN
;
lian Si FANG
Author Information
1. 中山大学附属第六医院
- Keywords:
Radiation injury;
α2-macroglobulin;
Human bone marrow mesenchymal stem cells;
Osteogenic differentiation
- From:
Chinese Journal of Pathophysiology
2017;33(11):2032-2037
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effect of α2-macroglobulin(α2M) against X-ray induced obstacle on osteo-genic differentiation of human bone marrow mesenchymal stem cells(hBMMSCs). METHODS:hBMMSCs were cultured in vitro. The 4th generation of hBMMSCs was irradiated with 8 Gy X-ray,then induced osteogenic differentiation and trea-ted with different concentrations of α2M(0.5 and 1.0 g/L). The alkaline phosphatase(ALP) activity and the mRNA ex-pression of runt-related transcription factor-2 (RUNX2) were detected on day 7 after osteogenic induction. The protein ex-pression of osteoglycin (OGN) was evaluated by Western blot on day 14 after osteogenic induction. The formation of calci-um nodules was detected by alizarin red staining on day 21 after osteogenic induction. The activity of superoxide dismutase (SOD) and the protein expression of MnSOD of irradiated hBMMSCs with 8 Gy X-ray were determined at 24 h after α2M treatment. RESULTS:Compared with 8 Gy X-ray group,the activity of ALP,the mRNA expression of RUNX2,the pro-tein expression of OGN and MnSOD,as well as SOD activity were higher than those in the hBMMSCs treated with α2M at 0.5 and 1.0 g/L after 8 Gy X-ray irradiation,and the calcium nodules were also increased. CONCLUSION:α2M signifi-cantly improves the osteogenic differentiation ability,the SOD activity and MnSOD protein expression of hBMMSCs after ra-diation injury.
