The neuroprotective effects of Fasudil on brain ischemia/reperfusion injury through maintaining blood-brain barrier function
10.3760/cma.j.issn.1674-6554.2017.10.014
- VernacularTitle:法舒地尔对小鼠脑缺血再灌注损伤保护作用的血脑屏障机制
- Author:
Jianjie CHENG
1
;
Xitong YANG
;
Xiaoshan DU
;
Hongyang XU
;
Guangming WANG
Author Information
1. 大理大学第一附属医院神经外科
- Keywords:
Ischemia;
Fasudil;
Evans blue;
Albumin;
Blood brain barrier (BBB)
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(10):933-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effects of Fasudil in cerebral I/R injury in mice.Methods 51 C57BL/6J mice was divided into two groups,CMC treated group (n=26) and Fasudil treated group (n=25),randomly.The mice were treated with Fasudil (10 mg/kg) or CMC (0.5％ CMC 10 ml/kg) separately.Then the treated mice were subjected to 60 min of focal ischemia and 18 h reperfusion.The infarct volume of brain was analyzed by TTC staining with MCID image system.BBB permeability was assessed by Evans blue extravasation and albumin leakage which was detected by immuno-blotting assay.The activity of MMP9 was analyzed by zymography.Results The infarct volume in CMC group ((99.07±6.53) mm3) was larger than that in Fasudil group ((57.02±8.93) mm3),the difference was statistically significant (P＜0.01).The activity of MMP9 in the mice treated with Fasudil was lower than that in CMC group.Compared with the CMC group(albumin (2.95±0.77),Evans blue (5.15±0.24)),the albumin and Evans blue content in the Fasudil treated group (albumin (1.04±0.18),Evans blue (1.96±0.31))reduced significanctly(all P＜0.01).Conclusion Fasudil protects I/R damage by inhibiting the activity of MMP9 to maintain blood-brain barrier permeability.
