Immunosuppressive effects of honokiol in a mouse model of particulate matter 2.5 -induced asthma
10.3760/cma.j.issn.0254-5101.2017.11.005
- VernacularTitle:和厚朴酚对PM2.5暴露哮喘小鼠免疫应答加剧的抑制作用
- Author:
Huicong FU
1
;
Xiaoxia LU
;
Feng HAN
;
Yurong FANG
;
Jiali XU
;
Liqiong ZHANG
;
Qing DU
;
Zongqi DONG
Author Information
1. 430016,华中科技大学同济医学院附属武汉儿童医院呼吸内科
- Keywords:
Asthma;
Particulate matter;
Th17 cell;
Treg cell;
Honokiol
- From:
Chinese Journal of Microbiology and Immunology
2017;37(11):827-833
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-inflammatory and immunosuppressive effects of honokiol in a mouse model of particulate matter ( PM ) 2.5-induced asthma .Methods Female SPF BALB/c mice were randomly divided into five groups:normal saline group (group A), ovalbumin (OVA)-sensitized group ( group B), PM2.5-exposed+OVA-sensitized group ( group C), dexamethasone-treated group (group D) and honokiol-treated group (group E).All mice except those in group A were sensitized and challenged with OVA, and the mice in groups C, D and E were exposed to PM2.5 every two days since the first challenge.Samples of lung sections were stained with hematoxylin and eosin (HE) to observe in-flammatory infiltration.Bronchoalveolar lavage fluid (BALF) and PBMCs were collected from each mouse . Expression of RORγt and Foxp3 at mRNA level was detected by quantitative real-time PCR.Flow cytometry analysis was performed to measure the percentages of Th 17 and Treg cells.ELISA was performed to measure the levels of IFN-γ, IL-10 and IL-17 in the supernatants of cell culture .Results Compared with group B , group C showed an enhanced expression of RORγt at mRNA level, increased IL-17 level and up-regulated percentage of Th17 cells (all P<0.05), but a suppressed expression of Foxp3 at mRNA level, decreased IL-10 level and down-regulated percentage of Th17 cells (all P<0.05).No significant difference in the per-centage of Th1 cells or in the expression of Th 1-related cytokines was observed .The expression of RORγt at mRNA level, IL-17 level and the percentage of Th 17 cells were decreased in PM2.5-exposed mice upon honokiol intervention (all P<0.05), while the expression of Foxp3 at mRNA level, IL-10 level and the per-centage of Treg cells were increased after honokiol intervention (all P<0.05).Honokiol had similar efficacy to dexamethasone in the treatment of asthma .Conclusion Honokiol can alleviate airway inflammation in mice with PM2.5 exposure-induced asthma through regulating the percentages of Th 17 and Treg cells.
