Prevalence of Mycoplasma pneumoniae infection in children in Hengyang and comparison of three methods for genotyping
10.3760/cma.j.issn.0254-5101.2017.11.010
- VernacularTitle:衡阳地区儿童肺炎支原体流行状况及3种基因分型方法比较
- Author:
Lianxia LIU
1
,
2
;
421001 衡阳,南华大学附属第一医院输血科
;
Jinhong XIAO
;
Wei TIAN
;
Lan YU
;
Xiaoxing YOU
;
Yanhua ZENG
;
Cuiming ZHU
Author Information
1. 421001 衡阳,南华大学病原生物学研究所,特殊病原体防控湖南省重点实验室
2. 421001 衡阳,南华大学附属第一医院输血科
- Keywords:
Mycoplasma pneumoniae;
p1 gene;
Genotype
- From:
Chinese Journal of Microbiology and Immunology
2017;37(11):862-868
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prevalence of Mycoplasma pneumoniae ( Mp) infection in children in Hengyang from 2013 to 2016 and to analyze the p1 genotypes of the isolated Mp strains by using polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP) , nested polymerase chain reaction (nPCR) and rapid-cycle polymerase chain reaction (Rapid-Cycle PCR).Methods Throat swab samples of children with acute respiratory tract infection were collected from four hospitals in Hengyang , Hu-nan Province from 2013 to 2016.Mp strains in these samples were identified by PCR amplification of the 16S rRNA gene.PCR-RFLP, nPCR and Rapid-Cycle PCR were performed for Mp p1 genotyping in order to fur-ther analyze the genotypes of Mp strains circulating in Hengyang .Results A total of 109 clinical strains of Mp were identified from the 984 throat swab samples .The sensitivities of PCR-RFLP and nPCR for genoty-ping MP strains were both 100％, while that of rapid-Cycle PCR was 98 .17％.All of the three methods showed 100％specificity for genotyping.Of all isolated Mp strains, 78.90％ were p1 gene type Ⅰ and 21.10％were p1 gene typeⅡ(t=93.239, P=0.01).From 2013 to 2016, the annual isolation rates of p1 gene type Ⅰ and type Ⅱ strains were 93.10％, 87.5％, 76.92％, 65.79％ and 6.90％, 12.5％, 23.08％, 34.21％, respectively.The rate of Mp p1 gene type Ⅰinfection decreased over year , while that of p1 gene type Ⅱinfection increased gradually .Conclusion PCR-RFLP, nPCR and rapid-Cycle PCR are reliable for genotyping of Mp p1 gene.The predominant genotype of Mp strains circulating in Hengyang is p 1 gene type Ⅰ, but the incidence of p 1 gene type Ⅱinfection gradually increases from 2013 to 2016 .
