To study the anti-inflammatory effect and recurrent oral ulcer model in rats of compound berberineXiaochuang powder
10.3760/cma.j.issn.1673-4246.2018.01.010
- VernacularTitle:复方黄连消疮灵的抗炎作用及其对复发性口腔溃疡模型大鼠炎症的影响
- Author:
Zhicheng YANG
1
;
Lifang WU
;
Junli ZHANG
;
Jiantao LYU
Author Information
1. 264100,滨州医学院烟台附属医院口腔科
- Keywords:
Oral ulcer;
Recurrence;
Compound berberine Xiaochuang powder;
Inflammation;
Mice;
Rats
- From:
International Journal of Traditional Chinese Medicine
2018;40(1):38-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of Compound berberineXiaochuangpowder on recurrent oral ulcers,and the anti-inflammatory effect.Methods The mice were divided into blank group, model group, and compound yellow hyalensis with high, medium, low dose group(2, 1, 0.5 g/kg equivalent medicine) and ibuprofen group(0.15 g/kg). Using mouse ear inflammation test by Xylene, To establish recurrent oral ulcer model by immunoassay in Rats, the mold SD rats according to random number table method is divided into blank group, model group, compound coptis sores spirit high, medium and low dose group (1, 0.5, 0.25 g/kg equivalent medicine), levamisole group (20 mg/kg), each group all gastric gavage corresponding drugs, model group, the blank group gastric gavage capacity such as pure water, a total of 30 days, observe the number of ulcers, duration and interval time, and the effection on serum IL-4, IL-8, VEGF level.Results The compound berberineXiaochuang powder high dose may significantly reduce the swelling degree of ears (7.46 ± 1.41 mg, 9.85 ± 2.71 mg vs. 16.25 ± 3.34 mg,P<0.05), reducing the number of ulcers (4.70 ± 1.27, 6.90 ± 3.70 vs. 9.90 ± 5.13,P<0.05), reduce ulcer duration (2.20 ± 1.22 d, 3.00 ± 1.05 d vs. 5.00 ± 1.15 d,P<0.05), prolonged intervals ulcers (8.00 ± 1.63 d, 5.00 ± 1.82 d vs. 2.40 ± 1.26 d,P<0.05). Compound berberineXiaochuang powder high dose can be significantly increased the level of serum IL-4 (68.92 ± 10.58 pg/ml vs. 55.18 ± 6.99 pg/ml, P<0.05) and lowered serum IL-8 (399.86 ± 62.14ng/mlvs. 578.70 ± 72.23 ng/ml,P<0.05); The compound berberine Xiaochuang powder high dose could significantly increase the expression of VEGF (41.41 ± 3.80 pg/ml、37.92 ± 3.48 pg/ml vs. 32.01 ± 4.32 pg/ml,P<0.05).Conclusions The compound berberineXiaochuang powder can increased the level of serum IL-4 and VEGF, reduced the level of IL-8, so as to achieve the treatment of recurrent oral ulcers.
