Digital gene expression profiling of dendritic cells treated with Seabuckthorn favones
10.3760/cma.j.issn.0254-5101.2017.11.007
- VernacularTitle:沙棘黄酮对树突状细胞免疫活性影响的数字基因表达谱分析
- Author:
Bingchun LIU
1
;
Ying QIU
;
Ruidong ZHAO
;
Xia HAN
;
Feiyu YUN
;
Erxia ZHOU
;
Sheng YUN
Author Information
1. 内蒙古医科大学附属医院干细胞研究中心
- Keywords:
Seabuckthorn flavone;
Dendritic cell;
Digital gene expression profile ( DGE);
Immu-noregulation
- From:
Chinese Journal of Microbiology and Immunology
2017;37(11):840-848
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes in morphology , phenotypes and gene expression pro-files of dendritic cells (DCs) following treatment with Seabuckthorn flavones (SF).Methods DCs were treated with 200μg/ml of SF and then cultured for 7 days.Changes in the morphology of DCs were observed under light microscope .Flow cytometry was used to detect DC surface molecules .Total RNA was extracted to construct the library for digital gene expression profiling ( DGE ) .Differentially expressed genes were screened out and further analyzed by gene ontology ( GO) enrichment analysis and Kyoto encyclopedia of genes and genomes ( KEGG ) pathway enrichment analysis .Results Compared with control group , SF treatment significantly enhanced the expression of HLA-DR, CD80, CD83 and CD86 on DCs.A total of 355 differentially expressed genes were screened out by DGE , including 176 up-regulated genes and 179 down-regulated genes .GO enrichment was mainly involved in the regulation and development of the immune sys -tem and other biological processes .KEGG pathway analysis showed that the significantly enriched pathways were closely related to inflammation , the immune system, cancer and other diseases .Conclusion SF can promote the expression of DC co-stimulatory molecules and pro-mature molecules, and regulate the expres-sion of immunity-related genes such as CD11a, SLAMF6, LMCD1, TSC22D3 and IKZF3.
