Effect of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice
10.3760∕cma.j.issn.0254-1416.2017.10.011
- VernacularTitle:GLYX-13对小鼠长时间异氟醚麻醉后认知功能的影响
- Author:
Huan LIU
1
;
Fangxia XU
;
Yin CUI
;
Tianjiao XIA
;
Peng ZHANG
;
Xiaoping GU
;
Zhengliang MA
Author Information
1. 210008,南京医科大学鼓楼临床医学院
- Keywords:
Excitatory amino acid agonists;
Isoflurane;
Cognition disorders
- From:
Chinese Journal of Anesthesiology
2017;37(10):1196-1200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of GLYX-13 on cognitive function after long-time isoflurane anesthesia in mice. Methods A total of 192 healthy male C57∕B6J mice, aged 8 weeks, weig-hing 22-25 g, were divided into 4 groups(n=48 each)using a random number table: control group (group C), isoflurane anesthesia group(group I), GLYX-13 group(group G), and isoflurane anesthesia plus GLYX-13 group(group IG). The animals were exposed to 15% isoflurane for 6 h in I and IG groups. GLYX-13 1 mg∕kg was injected via the caudal vein at 2 h before anesthesia in G and IG groups. Novel ob-ject recognition test and contextual fear conditioning test were performed on 1st, 3rd and 7th days after an-esthesia. The expression of 2B subunits-containing NMDA receptor(NR2B)and cyclic adenosine mono-phosphate response element-binding protein(CREB)mRNA in the hippocampus was detected by quantita-tive real-time polymerase chain reaction after the end of behavioral tests on 1st, 3rd and 7th days after anes-thesia. Results Compared with group C, the percentage of time spent in exploring a novel object, dis-crimination index and percentage of freezing time were significantly decreased, and the expression of NR2B and CREB mRNA in the hippocampus was down-regulated in group I(P <005). Compared with group I, the percentage of time spent in exploring a novel object, discrimination index and percentage of freezing time were significantly increased, and the expression of NR2B and CREB mRNA in the hippocampus was up-regulated in group IG(P <005). Conclusion GLYX-13 can significantly improve the cognitive func-tion after long-time isoflurane anesthesia in mice.
