Effect of sevoflurane postconditioning on microtubule structure during myocardial ischemia-reperfu-sion in mice
10.3760∕cma.j.issn.0254-1416.2017.10.012
- VernacularTitle:七氟醚后处理对小鼠心肌缺血再灌注时微管结构的影响
- Author:
Hai GUO
1
;
Min ZHENG
;
Pan ZHAO
;
Haiping MA
;
Hong ZHENG
Author Information
1. 新疆医科大学第一附属医院麻醉科
- Keywords:
Anesthetics;
inhalation;
Ischemic postconditionning;
Myocardial reperfusion inju-ry;
Microtubules
- From:
Chinese Journal of Anesthesiology
2017;37(10):1201-1203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane postconditioning on microtubule struc-ture during myocardial ischemia-reperfusion(I∕R)in mice. Methods Thirty-two healthy male C57 mice, aged 7-8 weeks, wighing 20-30 g, were divided into 4 groups(n=8 each)using a random number table:sham operation group(Sham group), group I∕R, sevoflurane postconditioning group(group SP), and mi-crotubule depolymerizer colchicine plus sevoflurane postconditioning group(group CSP). Myocardial I∕R was produced by occlusion of the left anterior descending branch of coronary artery for 30 min followed by 120 min reperfusion in I∕R, SP and CSP groups. Colchicine 004 μmol∕kg was intraperitoneally injected at 30 min before ischemia in group CSP. In SP and CSP groups, 24% sevoflurane was inhaled for 15 min starting from onset of reperfusion. Mice were sacrificed at 120 min of reperfusion, and myocardial speci-mens were obtained for determination of myocardial infarct size and expression of α-tubulin(by Western blot). Results Compared with group Sham, the myocardial infarct size was significantly increased, and the expression of α-tubulin was down-regulated in the other three groups(P<005). Compared with group I∕R, the myocardial infarct size was significantly decreased, and the expression of α-tubulin was up-regula-ted in group SP, and no significant change was found in the parameters mentioned above in group CSP(P>005). Compared with group SP, the myocardial infarct size was significantly increased, and the expres-sion of α-tubulin was down-regulated in group CSP(P<005). Conclusion The mechanism by which sevoflurane postconditionning reduces myocardial I∕R injury is related to stabilizing microtubule structure of mice.
