Effect of preconditioning with penehyclidine hydrochloride on mitochondria-dependent pathway of apoptosis during myocardial ischemia-reperfusion in rats
10.3760∕cma.j.issn.0254-1416.2017.10.029
- VernacularTitle:盐酸戊乙奎醚预先给药对大鼠心肌缺血再灌注时线粒体调控凋亡通路的影响
- Author:
Hongbao TAN
1
;
Duomao LIN
;
Jun MA
Author Information
1. 410006,长沙市第四医院麻醉科 长沙市消化外科研究所
- Keywords:
Cholinergic antagonists;
Apoptosis;
Myocardial reperfusion injury
- From:
Chinese Journal of Anesthesiology
2017;37(10):1267-1270
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of preconditioning with penehyclidine hydrochloride (PHC)on mitochondria-dependent pathway of apoptosis during myocardial ischemia-reperfusion(I∕R)in rats. Methods Twenty-four healthy adult male Wistar rats were allocated into 3 groups(n=8 each)using a random number table: sham operation group(group S), I∕R group and PHC preconditioning group (group P). Myocardial I∕R was produced by occlusion of left anterior descending branch of coronary artery for 30 min followed by 3 h reperfusion. PHC 10 mg∕kg was injected via the tail vein at 30 min before oc-clusion in group P. The rats were sacrificed at the end of reperfusion, hearts were removed and left ventric-ular specimens were obtained for determination of apoptosis in cardiomyocytes(by TUNEL), and apoptosis index(AI)was calculated. Mitochondria and cytoplasm were isolated for detection of mitochondrial mem-brane potential(MMP, by JC-1 method)and expression of Bcl-2, cytochrome c(Cyt c)in mitochondri-a and cytoplasm and activated caspase-3 in myocardial tissues(by Western blot). Results Compared with group S, AI was significantly increased, the expression of Bcl-2 and mitochondrial Cyt c was down-regula-ted, MMP was decreased, and the expression of Cyt c in cytoplasm and activated caspase-3 was up-regula-ted in I∕R and P groups(P<0. 05). Compared with group I∕R, AI was significantly decreased, the ex-pression of Bcl-2 and mitochondrial Cyt c was up-regulated, MMP was increased, and the expression of Cyt c in cytoplasm and activated caspase-3 was down-regulated in group P(P<0. 05). Conclusion The mechanism by which PHC preconditioning attenuates myocardial I∕R injury is related to inhibiting mitochon-dria-dependent pathway of apoptosis in rats.
