Analysis of clinical outcomes of different embryo stage biopsy in array comparative genomic hybridization based preimplantation genetic diagnosis and screening
10.3760/cma.j.issn.0529-567x.2017.12.007
- VernacularTitle:基于微阵列比较基因组杂交技术的胚胎植入前遗传学诊断和筛查在不同阶段胚胎中的临床应用结局分析
- Author:
Jiandong SHEN
1
;
Wei WU
;
Li SHU
;
Lingbo CAI
;
Jiazi XIE
;
Long MA
;
Xueping SUN
;
Yugui CUI
;
Jiayin LIU
Author Information
1. 210029,南京医科大学第一附属医院生殖医学中心生殖医学国家重点实验室
- Keywords:
Preimplantation diagnosis;
Comparative genomic hybridization;
Microarray analysis;
Cleavage stage;
ovum;
Blastula;
Biopsy;
Preimplantation genetic screening
- From:
Chinese Journal of Obstetrics and Gynecology
2017;52(12):828-834
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficiency of the application of array comparative genomic hybridization (array-CGH) in preimplantation genetic diagnosis or screening (PGD/PGS), and compare the clinical outcomes of different stage embryo biopsy. Methods The outcomes of 381 PGD/PGS cycles referred in the First Affiliated Hospital of Nanjing Medical University from July 2011 to August 2015 were retrospectively analyzed. There were 320 PGD cycles with 156 cleavage-stage-biopsy cycles and 164 trophectoderm-biopsy cycles, 61 PGS cycles with 23 cleavage-stage-biopsy cycles and 38 trophectoderm-biopsy cycles.Chromosomal analysis was performed by array-CGH technology combined with whole genome amplification.Single embryo transfer was performed in all transfer cycles.Live birth rate was calculated as the main clinical outcomes. Results The embryo diagnosis rate of PGD/PGS by array-CGH were 96.9%-99.1%. In PGD biopsy cycles, the live birth rate per embryo transfer cycle and live birth rate per embryo biopsy cycle were 50.0%(58/116) and 37.2%(58/156) in cleavage-stage-biopsy group, 67.5%(85/126) and 51.8%(85/164) in trophectoderm-biopsy group (both P<0.01). In PGS biopsy cycles, the live birth rate per embryo transfer cycle and live birth rate per embryo biopsy cycle were the same as 34.8%(8/23) in cleavage-stage-biopsy group, the same as 42.1%(16/38) in trophectoderm-biopsy group (both P>0.05). Conclusions High diagnosis rate and idea live birth rate are achieved in PGD/PGS cycles based on array-CGH technology.The live birth rate of trophectoderm-biopsy group is significantly higher than that of cleavage-stage-biopsy group in PGD cycles;the efficiency of trophectoderm-biopsy is better.
