The influence of the transfection of SDF-1αto the heart mediated by recombinant adenovirus ,microbubbles and ultrasound on non targeted tissues of rats with acute myocardial infarction
10.3760/cma.j.issn.1004-4477.2017.11.018
- VernacularTitle:超声 、 微泡及重组腺病毒联合介导外源性基质细胞衍生因子1α 转染急性心肌梗死大鼠心脏对非靶组织的影响
- Author:
Gaofeng SU
1
;
Liyun LIU
;
Yuming MU
Author Information
1. 新疆医科大学第一附属医院心脏超声诊断科
- Keywords:
Ultrasound targeted microbubble destruction;
Acute myocardial infarction;
SDF-1α;
Gene transfection in non-target tissue
- From:
Chinese Journal of Ultrasonography
2017;26(11):996-1000
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate gene transfection in liver , lung and kidney by ultrasound , microbubble and recombinant adenovirus mediated exogenous stromal cell derived factor-1α ( SDF-1α) gene transfer to the heart in rats with acute myocardial infarction( AMI) . Methods Forty AMI SD rats were randomly divided into control and experimental groups:myocardial infarction + ultrasound irradiation group (M+ U/control group , n = 10) ,and 3 experimental subgroups on the basis of pAd-EGFP/SDF-1α ( The biotin recombinant adenovirus expressing enhanced green fluorescent protein and SDF-1α) . Genes transfection length of time:1 day ,2 days and 3 days of transfection ( M +S1+U ,M+S2+U and M+S3+U group) . The expression of EGFP in liver ,lung and kidney were detected by laser scanning confocal microscopy at 7 days after administration . Results There was a little expression of EGFP in the liver ,lung and kidney in the drug delivery group and no expression in the control group .The differences in the expression of EGFP between all the gene delivery groups and the control group were statistically significant ( P <0 .05) . With the increase of the number of medication days , the target gene transfection increased slightly ,but there was no significant difference among the different drug delivery groups . Conclusions When the ultrasound ,microbubble and recombinant adenovirus mediated exogenous SDF-1αgene transfer to the heart in AMI rats ,liver ,lung and kidney tissues will also be transfected . However ,with the increasing of the days of administration , the transfection of target gene in non-target tissue produces only a slight accumulation . The transfection area of target gene in non-target tissue is not linear correlated with the days of administration .
