Effect of Niaogan Decoction on expulsion of uropathogenic Escheriehia coli from human bladder epithelial cells
10.3969/j.issn.1001-1528.2017.12.005
- VernacularTitle:尿感方清除受感染膀胱上皮细胞胞内尿道致病性大肠杆菌的作用
- Author:
Yu WU
1
;
Jian JIANG
;
Min HE
;
yang Lei ZHANG
;
weng Weng CHEN
Author Information
1. 上海中医药大学附属曙光医院临床药理科
- Keywords:
Niaogan Decoction;
UPEC;
expulse;
human urinary bladder epithelial cell;
TLR4/cAMP signal pathway
- From:
Chinese Traditional Patent Medicine
2017;39(12):2469-2474
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the effects of Niaogan Decoction (Portulacae Herba,Taraxaci Herba) on expulsion of uropathogenic Escheriehia coli (UPEC) from human bladder epithelial cells and to explore its mechanism.METHODS After HTB-9 was infected with UPEC,drug urine exocytosed the UPEC invaded into urinary bladder epithelial cell were observed.With molecular biology technology,the mechanism of key factors to TLR4/cAMP signal pathway such as Toll-like receptor 4 (TLR4),adeuylate cyclase 3 (AC3),cyclic adenosine monophosphate (cAMP),protien kinase A (PKA),Myosin Ⅶ A and Rab interacting protein (MyRIP),Rab27b and Caveolin-1 were analyzed.RESULTS Treatment with Niaogan Decoction resulted in a significant increase in UPEC exocytosis compared with the blank control high dose group.Niaogan Decoction group improved TLR4,AC3,MyRIP,Rab27b and Caveolin-1 protein expression,increased intracellular cAMP content and promoted PKA activation.CONCLUSION Niaogan Decoction has the capacity to exocytose UPEC,its mechanism may relate to TLR 4/cAMP signal pathway.
