Quality standard for Weigela japonica var.sinica
10.3969/j.issn.1001-1528.2017.12.017
- VernacularTitle:水马桑质量标准的研究
- Author:
zhi Jun WANG
1
;
ling Bao GOU
;
qi Hong ZHANG
;
li Hai YU
;
ling Ai WANG
;
fang Hui L(U)
;
Kun ZOU
Author Information
1. 三峡大学天然产物研究与利用湖北省重点实验室
- Keywords:
Weigela japonica Thunb.var.sinica (Rehd.) Bailey (W.j.);
scopoletin;
total coumarins;
TLC;
HPLC;
UV
- From:
Chinese Traditional Patent Medicine
2017;39(12):2529-2533
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the quality standard for Weigela japonica Thunb.var.sinica (Rehd.) Bailey (W.j.).METHODS TLC was adopted in this medicinal material's qualitative identification after morphological identification and microscopic identification.The contents of water,total ash,acid-insoluble ash and extracts were detected according to Chinese Pharmacopoeia methods.Then the contents of scopoletin and total coumarins were determined by HPLC and UV,respectively.RESULTS The morphologies and microscopic characters of W.j.could be distinguished from other same generic plants.The clear TLC spot displayed good resolution.The contents of water,total ash,acid-insoluble ash,water-soluble extract and acid-soluble extract were no more than 12.0％,no more than 2.0％,no more than 0.5％,no less than 5.0％ and no less than 4.5％,respectively.Scopoletin and total coumarins showed good linear relationships within the ranges of 1.25-40.0 μg/mL(r =0.999 7)and 2.0-64.0 μg/mL (r =0.999 9),whose average recoveries were 98.19％ (RSD =0.90％) and 99.21％ (RSD =2.5％),respectively.CONCLUSION This accurate and reliable method can be used for the quality control of W.j..
