Establishment of HPLC fingerprints of Yupingfeng Powder aqueous decoction and determination of nine constituents
10.3969/j.issn.1001-1528.2017.12.022
- VernacularTitle:玉屏风散水煎液HPLC指纹图谱的建立及9种成分测定
- Author:
Hui DING
1
;
wei Wei GU
;
Lü JI
;
rong Lu ZHANG
;
rong Guo JIANG
Author Information
1. 南京中医药大学药学院
- Keywords:
Yupingfeng Powder aqueous decoction;
fingerprints;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(12):2550-2554
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC fingerprints of Yupingfeng Powder aqueous decoction and to determine the contents of nine constituents.METHODS The analysis of aqueous decoction was developed on a 30 ℃ thermostatic Hypersil ODS column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 220 nm.RESULTS There were fifteen common peaks in the fingerprints of ten batches of samples,with the similarities of more than 0.95.Nine of them were identified as prim-O-glucosylcimifugin,calycosin-7-O-β-D-glucoside,cimifugin,4'-O-β-glucopyranosyl-5-O-methylvisamminol,psoralen,calycosin,sec-O-glucosylhamaudol,formononetin and atractylon,which showed good linear relationships within their own ranges (r ≥ 0.999 7),the average recoveries were 97.91％-99.81％ with the RSDs of 0.58％-1.27％.CONCLUSION This stable and reliable method can beused for the quality control of Yupingfeng Powder aqueous decoction.
