Simultaneous determination of two constituents in five parts of Belladonnae Herba by RP-HPLC
10.3969/j.issn.1001-1528.2017.12.024
- VernacularTitle:RP-HPLC法同时测定颠茄草5个部位中2种成分
- Author:
Guo ZHUANG
1
;
qing Yu WANG
;
Wei XIONG
;
Lei LI
Author Information
1. 南阳医学高等专科学校博士后研发基地
- Keywords:
Belladonnae Herba;
atropine sulfate;
scopolamine;
parts;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(12):2560-2563
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an RP-HPLC method for the simultaneous content determination of two constituents in five parts (whole grass,roots,stems,leaves and fruits) of Belladonnae Herba.METHODS The analysis of Belladonnae Herba methanol extract was carried out on a 35 ℃ thermostatic Agilent TC-C18 column (4.6 mm × 150 mm,5 μm),with the mobile phase comprising of acetonitrile-phosphate buffer flowing at 1.0 ml/min in an isocratic elution manner,and the detection wavelength was set at 216 nm.RESULTS Atropine sulfate and scopolamine hydrobromide showed good linear relationships within the ranges of 51.60-1 290 mg/L (R2 =0.999 9)and 2.90-72.00 mg/L (R2 =0.999 5),whose average recoveries were 101.3％ and 101.5％ with the RSDs of 2.5％ and 1.3％,respectively.The contents of two constituents were the highest in the roots and leaves,respectively,and both of them demostrated the lowest contents in the stems.CONCLUSION The contents of atropine sulfate and scopolamine in different parts of Belladonnae Herba show obvious differences.
