Effects of sevoflurane on expression of Egr-1 and Egr-2 in suprachiasmatic nuclei of sleep-deprived rats
10.3760/cma.j.issn.0254-1416.2017.09.018
- VernacularTitle:七氟醚对睡眠剥夺大鼠视交叉上核Egr-1和Egr-2表达的影响
- Author:
Qianni SHEN
1
;
Jiabao HOU
;
Bo ZHAO
;
Yang WU
;
Lian LIU
;
Qingtao MENG
;
Zhongyuan XIA
Author Information
1. 430060,武汉大学人民医院麻醉科
- Keywords:
Anesthetics,inhalation;
Sleep deprivation;
Optic chiasm;
Chronobiology disorders
- From:
Chinese Journal of Anesthesiology
2017;37(9):1102-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of sevoflurane on the expression of early growth response gene 1 (Egr-1) and Egr-2 in the suprachiasmatic nuclei (SCN) of sleep-deprived rats.Methods Forty-eight pathogen-free healthy male Sprague-Dawley rats,were divided into 4 groups (n =12 each) using a random number table:control group (group C),sleep deprivation group (group SD),sevoflurane group (group SEV) and sleep deprivation plus sevoflurane group (group SD+SEV).The rats were subjected to sleep deprivation for 96 h in group SD.The rats inhaled 2.5％ sevoflurane for 3 h in group SEV.The rats inhaled 2.5％ sevoflurane for 3 h after being subjected to sleep deprivation for 96 h in group SD+SEV.The mechanical paw withdrawal threshold (MWT) was measured at 12 h,3 days and 7 days after emergence from anesthesia (T1-3).The animals were sacrificed after blood samples were obtained from the external carotid artery,and the cerebral SCN was removed for determination of Egr-1 and Egr-2 expression by Western blot.Results Compared with group C,the MWT was significantly decreased at T1-3 in SD,SEV and SD+SEV groups,the expression of Egr-1 and Egr-2 in SCN was significantly up-regulated at T1-3 in SD and SD+SEV groups,and the expression of Egr-1 in SCN was significantly up-regulated at T1-3,and the expression of Egr-2 in SCN was up-regulated at T1 in group SEV (P＜0.05).Compared with SD and SEV groups,the MWT was significantly decreased at T1-3 in group SD+SEV,and the expression of Egr-1 and Egr-2 in SCN was significantly up-regulated at T1-3 in group SD+SEV (P＜0.05).Conclusion The mechanism by which sevoflurane aggravates hyperalgesia is related to up-regulation of Egr-1 and Egr-2 expression in SCN of sleepdeprived rats.
