Simultaneous determination of somedon and its metabolites by GC–MS/MS

VernacularTitle:生物样品中去痛片4种成分及其8种代谢物的GC-MS/MS法检测
Author: Yanan SHEN ¹ ; Juan JIA ; Feng ZHENG ; Aiai FAN ; Zhiwen WEI ; Yao LIU ; Shanlin FU ; Keming YUN
Author Information

1. 山西医科大学法医学院
Keywords: forensic toxicological analysis; somedon; Supported liquid extraction; GC-MS/MS
From: Chinese Journal of Forensic Medicine 2017;32(6):573-577,587
CountryChina
Language:Chinese
Abstract: Objective To develop a method of support liquid-liquid extraction (SLE) and simultaneous determination of 4 components in somedon and its 8 metabolites by GC–MS/MS. Methods Somedon and its metabolites were extracted by SLE and determined by GC-MS/MS in MRM mode. The qualitative analysis was based on retention time and ratio of ions. The quantitative analysis was based on internal standard method and calibration curve. Results After SLE and determination of 4 components in somedon and its 8 metabolites, the extraction rate were 37.57%~95.87%, the linear range were 0.12μg/mL~16.00μg/mL, the correlation coefficient(r)were 0.989 6~0.999 7, LOD were 0.08ng/mL~14.48ng/mL, the accuracy were 79.63%~122.90%, the interday and intraday precision were 0.99%~7.43% and 2.19%~10.60% respectively. Conclusion Simultaneous determination of somedon and its metabolites by GC–MS/MS in biological samples, which was rapid, simple, accurate and was high precision and recovery, can be used for qualitative and quantitative analysis in forensic cases.