Study on the preparation method of capillary electrophoresis sieving medium based on domestic genetic analyzer
10.13618/j.issn.1001-5728.2017.06.017
- VernacularTitle:基于国产遗传分析仪的毛细管电泳筛分介质的制备
- Author:
Lijuan YU
1
;
Yanchai NIE
;
Bowei JIANG
;
Ying ZHAO
;
Tao ZHANG
;
Chuan JIN
Author Information
1. 公安部第一研究所
- Keywords:
forensic biological evidence;
capillary electrophoresis;
sieving medium;
domestic genetic analyzer;
STR typing
- From:
Chinese Journal of Forensic Medicine
2017;32(6):618-622
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a capillary electrophoresis sieving medium and apply it in GA118-16A genetic analyzer. Methods The white solid polyacrylamide (LPA) was prepared by polymerization and lyophilized. Through the swelling of the sol buffer, the sieving medium was obtained. The sieving medium was evaluated by 1) characterizing the parameters, including molecular weight, structure and viscosity, 2) applying in the GA118-16A genetic analyzer, including the spatial calibration, the spectral calibration and the STR analysis.. Results The prepared sieving medium Mw 1.8 x 105Da, Mn 1.2 x 105 Da, is of correct structure and high purity. The polydispersity was 1.5The spatial calibration and spectral calibration files can be established successfully in GA118-16A genetic analyzer, and the sieving medium can effectively separate the DNA fragments with 1bp difference. The STR profile is of sharp peaks, no impurity peaks, no tail, and no peak loss. Conclusion The sieving medium prepared by the method can be applied to domestic genetic analyzer such as GA118-16A.
