Identification of Brucella strain isolated from sheep blood in Jingyuan County Gansu Province
10.3760/cma.j.issn.2095-4255.2017.12.004
- VernacularTitle:甘肃省靖远县绵羊血液内分离的布鲁菌菌株种型鉴定
- Author:
Wu LIU
1
;
Liansheng LI
;
Buyun CUI
;
Deshan YU
;
Jiusheng WEI
;
Shubo YANG
;
Liguo YANG
;
Qiaoling ZHOU
Author Information
1. 甘肃省靖远县疾病预防控制中心传染病防制科
- Keywords:
Brucella;
Melitensis;
Identification
- From:
Chinese Journal of Endemiology
2017;36(12):870-873
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the Brucella strains isolated from sheep blood in Jingyuan County Gansu Province,and to provide scientific basis for Brucellosis control and prevention.Methods In 2016,blood samples from suspected cases of sheep were collected in the home of Brucellosis patient of Jingyuan County,and cultured with vaccination double blood bottles via the enrichment method,and the Brucella strains isolated from sheep were identified by traditional bio-typing,genus specific Brucella surface protein 31 PCR (BSCP31-PCR) and Brucella abortus,Brucella melitensis,Brucella ovis,Brucella suis species-specific PCR (AMOS-PCR),respectively.Results The isolated strain was identified using the traditional biological classification method,bacteria could grow in thionine and fuchsin dyes,A and M factors serum agglutination tests were positive,Bk2 phage lysis test was positive,but Tb and Wb phage lysis tests were negative;and the results of BSCP31-PCR of tested strain was 223 bp band same to Brucella;the results of AMOS-PCR was 731 bp band same to Brucella melitensis.Conclusion Conventional bio-typing test assisted with molecular techniques has confirmed that the isolated Brucella strain from sheep blood is Brucella melitensis type 3 in Jingyuan County Gansu Province.
