Comparison of forensic parameters between length-based and sequence-based STR models
10.13618/j.issn.1001-5728.2017.06.023
- VernacularTitle:长度多态与序列多态STR模型法庭科学参数比较
- Author:
Peng ZHAO
1
;
Guangfeng ZHANG
;
Jing LIU
;
Zhengliang YU
;
Zhen XU
;
Jian YE
;
Anquan JI
;
Le WANG
Author Information
1. 现场物证溯源技术国家工程实验室
- Keywords:
forensic genetics;
Short tandem repeat;
Length polymorphism;
Sequence polymorphism;
Next generation sequencing
- From:
Chinese Journal of Forensic Medicine
2017;32(6):642-644,648
- CountryChina
- Language:Chinese
-
Abstract:
Capillary electrophoresis-based STR genotyping is accepted as the gold standard for human individual identification. Next generation sequencing (NGS) allows for the full resolution of STR base composition, and has the potential to be widely used in the field of forensics. Compared with length polymorphism, STR sequencing could provide more information, and quantitatively calculating the forensic parameters is necessary. In this paper, we established simple models for length-based and sequence-based STRs, and calculated the forensic parameters for both models. The results showed that for a single STR locus, compared with length polymorphism, STR sequence polymorphism could provide higher power of discrimination and power of exclusion, indicating sequence-based STR marker have stronger ability for identifying unrelated individuals and exclude non biological father. By combining 15 non-linkage loci for forensic DNA analysis, the cumulative matching probability values for length-based and sequence-based STR models are at 10-18and 10-26levels, respectively. Only 10 non-linkage sequence-based STR is required to reach a cumulative matching probability of as high as 15 length-based STR loci. It is hoped that these simulated models and calculations can provide a reference for the forensic application of NGS-based STR genotyping.
