Effect of remifentanil on metastasis of human lung adenocarcinoma cells and expression of interleukin-7 receptors
10.3760/cma.j.issn.0254-1416.2017.09.008
- VernacularTitle:瑞芬太尼对人肺腺癌细胞转移能力和白细胞介素-7受体表达的影响
- Author:
Yuanbo WU
1
;
Xin LI
;
Shengqiang LU
;
Yan WU
;
Ming YU
Author Information
1. 湖北省肿瘤医院麻醉科
- Keywords:
Piperidines;
Lung neoplasms;
Neoplasm metastasis;
Receptors,interleukin-7
- From:
Chinese Journal of Anesthesiology
2017;37(9):1058-1060
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of remifentanil on the metastasis of human lung adenocarcinoma cells and expression of interleukin-7 receptor (IL-7R).Methods Human adenocarcinoma cell line A549 cells were seeded in 24-well plates at a density of 2× 105 cells/ml (72 wells).The cells were divided into 4 groups (n =18 each) using a random number table:normal control group (group C) and remifentanil 2,4 and 6 ng/ml groups (group R2,group R4,group R6).In R2,R4 and R6 groups,remifentanil at the final concentrations of 2,4 and 6 ng/ml was added,respectively,and the cells were incubated for 4 h.The equal volume of normal saline was given instead of remifentanil in group C.The cells were collected at 24 h after the following incubation.The invasion of cells was determined by Transwell invasion assay,and the invaded cells were counted.The migration of cells was determined by cell scratch test,and cell migration rates were calculated.The expression of IL-7R in cells was detected by Western blot.Results Compared with group C,the number of invaded cells and cell migration rates were significantly decreased in R2,R4 and R6 groups,and the expression of IL-7R was down-regulated in R4 and R6 groups (P＜0.05).Compared with group R2,the number of invaded cells and cell migration rates were significantly decreased,and the expression of IL-7R was down-regulated in R4 and R6 groups (P＜0.05).Compared with group R4,the number of invaded cells and cell migration rates were significantly decreased,and the expression of IL-7R was down-regulated in group R6 (P＜0.05).Conclusion Remifentanil can inhibit metastasis of human lung adenocarcinoma cells and down-regulate the expression of IL-7R.
