Protocol of dialysis-type processing on frozen platelets
10.7644/j.issn.1674-9960.2017.09.005
- VernacularTitle:冰冻血小板透析式洗涤方法研究
- Author:
Hui CAO
1
;
yang Xiao YI
;
xia Min LIU
;
yun Jian WANG
;
xi Jie WANG
;
Qun LUO
;
Ying HAN
Author Information
1. 军事医学科学院野战输血研究所
- Keywords:
frozen platelet;
washing machine;
cryopreservation;
quality evaluation
- From:
Military Medical Sciences
2017;41(9):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new dialysis washing method for human frozen platelets and to evaluate a new type of washing machine based on dialysis.Methods Twenty bags of platelets(1U)were divided into two groups:centrifugation group and dialysis group.The effect of the washing machine was evaluated by measuring platelet function in vitro.Results After the platelets were treated by the two methods, the recovery rate of dialysis group[(73.57 ± 12.49)%]was significantly better than that of centrifugal group[(62.21 ±7.07)%].The osmotic pressure of dialysis group was 305.8 ±5.27mOsm/kg, compared to 383.2 ±22.00 mOsm/kg in centrifugation group.There was significant differences in the recovery rate and osmotic pressure(P<0.05), but there was no significant difference between dialysis group and centrifugation group in the mean platelet volume[(9.44 ±0.93)vs(9.23 ±0.50)fl], ADP-induced aggregation rate[(20.7 ±8.00)%vs(27.7 ±11.83)%],thrombin-induced platelet aggregation rate[(84.1 ±7.88)%vs(92.9 ±5.28)%], hypotonic shock responses[(19.02 ±4.42)% vs(23.19 ±9.08)%], CD62p activation rate [(16.00 ±9.40)%vs(21.86 ±8.14)%]or apoptosis rate[(46.31 ±10.28)% vs(54.59 ±16.76)%](P>0.05). Conclusion Compared to centrifugation group,the frozen platelet washing machine can not only improve platelet recovery,but also solve the problem of excessive DMSO residues.
