Visualization of proliferation activity of luminescent bacteria in different physiological media
10.7644/j.issn.1674-9960.2017.09.016
- VernacularTitle:发光细菌在不同生理介质中增殖活性的可视化研究
- Author:
Jing LIU
1
;
Yu SUN
;
Hui LI
;
lin Chu HE
;
Ru WANG
;
Ling LI
;
zhong Hui WANG
;
hui Xiao WANG
Author Information
1. 解放军305医院
- Keywords:
Photobacterium;
Escherichia coli;
Pseudomonas aeruginosa;
single platelets;
plasma;
proliferation
- From:
Military Medical Sciences
2017;41(9):762-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the number of colonies and the luminous intensity of two kinds of platelets contamination bacteria,Escherichia coli(ECO)and Pseudomonas aeruginosa(PAE)in single platelets, plasma and phosphate buffered salines(PBS), and to explore their growth and proliferation in different biological fluid media.Methods The two luminescent bacteria were constructed before the correlations between the number of colonies and the luminous intensity in different media in vitro were observed with a small animal bioluminescence imaging instrument, and correlation analysis was carried out.By recording the luminous intensity at serial time points in different media, a growth curve was drown to reflect the proliferation of bacteria.Results There was a significant positive correlation between the number of colonies and luminous intensity of ECO and PAE in single platelets,plasma and PBS.The larger the number of colonies was,the stronger the luminous intensity was.The results indicated that single platelets significantly inhibited the proliferation of ECO and PAE,and that plasma also had some inhibitory effect,but not so strong.Conclusion For the two luminous bacteria,luminous intensity can represent the growth and proliferation of bacteria.Single platelets have obvious inhibitory effect on the growth of ECO and PAE, and the plasma also has some inhibitory effect, but the effect is not so strong as that of single platelets.
