Detection of four DNA genetic marker systems to differentiate individuals in mixed seminal stain of two individuals
10.13618/j.issn.1001-5728.2017.06.019
- VernacularTitle:四类遗传标记的检测用于混合精斑的个人识别
- Author:
Lu ZHANG
1
;
Mei DING
;
Hao PANG
;
Miao FAN
;
Jun YAO
;
Rui ZHANG
;
Jiaxin XING
;
Jinfeng XUAN
;
Ziqing LIN
;
Baojie WANG
Author Information
1. 中国医科大学法医学院
- Keywords:
forensic biological evidence;
STR;
mtDNA;
SSCP;
mixed seminal stains;
personal identification
- From:
Chinese Journal of Forensic Medicine
2017;32(6):627-630
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate personal identification of mixed seminal stain of two individuals, we combined the detection of genotyping autosomal, Y and X STR and sequencing mtDNA hypervariable Ⅰ (HV Ⅰ ) region. Methods We analyzed autosomal, Y and X STR with commercial kit and separating and sequencing HVⅠfragments of mixed seminal stain from two males by SSCP electrophoresis. Results Four genetic markers of the high amount sample can be obtained when mixed ratio is more than 1:10. When the proportion of two samples is close, the suspect could be excluded or, to some extent, identified by comparing with our results. Conclusion The combined detection of four genetic marker systems can, to some degree, solve the personal identification from mixed seminal stain of two individuals.
