Interleukin-1 beta, -6 and interferon-gamma productions in patients undergoing continuous ambulatory peritoneal dialysis.
10.3349/ymj.1998.39.3.240
- Author:
Kyu Hun CHOI
1
;
In Hee LEE
;
Sug Kyun SHIN
;
Hyun Jin NOH
;
Shin Wook KANG
;
Ho Young LEE
;
Dae Suk HAN
Author Information
1. Department of Internal Medicine, the Institute of Kidney Disease, Yonsei University, College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Interleukin-1;
interleukin-6;
interferon-;
CAPD
- MeSH:
Adult;
Cells, Cultured;
Female;
Human;
Interferon Type II/biosynthesis*;
Interleukin-1/biosynthesis*;
Interleukin-6/biosynthesis*;
Male;
Middle Age;
Monocytes/metabolism;
Parathyroid Hormones/blood;
Peritoneal Dialysis, Continuous Ambulatory*;
Serum Albumin/analysis
- From:Yonsei Medical Journal
1998;39(3):240-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to investigate cytokine productions in patients undergoing continuous ambulatory peritoneal dialysis (CAPD), we studied the production of interleukin (IL)-1 beta, -6 and interferon (IFN)-gamma by cultured peripheral blood mononuclear cells (PBMC) in peritonitis-free CAPD patients. The correlation of cytokine production with plasma parathyroid hormone (PTH) and albumin levels was also evaluated. While the release of IL-1 beta was not markedly different from controls release of IL-6 from 24-hour cultured PBMCs was significantly greater than that of controls, (Mean +/- S.D., IL-6: 2186.8 +/- 1217.9 pg/ml, vs 1516.3 +/- 767.9, P <0.05). The addition of lipopolysaccharide (LPS, 10 micrograms/ml, significantly stimulated IL-1 beta and -6 production of PBMCs in CAPD patients and controls, compared to an unstimulated condition. The LPS-induced IL-1 beta production was also not markedly different from controls, whereas LPS-induced IL-6 production was significantly higher than controls (IL-6: 13,220.7 +/- 7177.4 vs 7411.4 +/- 1236.9, P <0.05). However, the percentage increases of IL-6 production stimulated with LPS in CAPD patients were not significantly different from controls (p > 0.05). No difference of baseline IFN-gamma was detected between CAPD patients controls, but phytohemagglutinin (PHA, 10 micrograms/ml)-stimulated IFN-gamma release was significantly higher in CAPD patients than controls (2425.9 +/- 1565.0 pg/ml vs 1364.0 +/- 755.1, P <0.05). There was no significant correlation between PTH and, IL-1 beta, serum albumin level and LPS-stimulated IL-6 production (r = 0.54, P <0.05). In conclusion, CAPD seems to partly induce activation of PBMCs with an enhanced release of IL-6 and IFN-gamma, and CAPD patients with higher serum albumin levels tend to show higher IL-6 production in immune response.
