Effects of piogiltazone on the expression of hemooxygenase-1 in aortic atherosclerosis plaque area in rabbits
10.7652/jdyxb201801006
- VernacularTitle:盐酸吡咯列酮对血红素氧合酶-1在动脉粥样硬化模型兔主动脉表达的影响
- Author:
qiang Jun PAN
1
;
xin Dian ZHANG
;
feng Chao SUN
;
Jin YANG
Author Information
1. 西北大学生命科学学院预防基因组医学研究所
- Keywords:
hemooxygenase-1;
pioglitazone;
atherosclerosis;
aorta;
rabbit
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2018;39(1):22-25,115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of piogiltazone on the expression of hemooxygenase-1 in aortic atherosclerosis plaque area in rabbits .Methods We randomly divided 36 Watanabe heritable hyperlipidemic rabbits into 3 groups:control group ,high-cholesterol diet group ,and high-cholesterol diet with pioglitazone [8 mg/(kg · d)] group ,with 12 in each .All the animals were fed for 12 weeks and then sacrificed .Rabbit aortic atherosclerosis pathological features were observed by Masson staining ;the expression of hemooxygenase-1 in aortic atherosclerosis plaque area in rabbits was detected by immunohistochemistry , real-time quantitative PCR and Western blot techniques .Results Hemooxygenase-1 expression was obvious in the smooth muscle in aortic vessels and atherosclerosis plaques in the three groups of rabbits . The expression of hemooxygenase-1 was lower in high-cholesterol diet with pioglitazone group than in high-cholesterol diet group and significantly higher than in control group (P<0 .05) .Conclusion Pioglitazone can slow down the progression of atherosclerosis by up-regulating the expression of hemooxygenase-1 .
