Effects of NO on T lymphocyte subsets in peripheral blood of spontaneously hypertensive rats
- VernacularTitle:一氧化氮对自发性高血压大鼠外周血T淋巴细胞亚群的影响
- Author:
wang Tu SHEN
1
;
ya Li SHAN
;
shi Xiu YU
;
Min PENG
;
Xin NI
;
Li LI
;
qiang Jun SI
;
zhi Xin LI
;
tao Ke MA
Author Information
- Keywords: spontaneously hypertensive rat; T lymphocyte; NO; TNF-α
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2018;39(1):26-30
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate whether NO can relieve hypertensive cerebrovascular and renal injury by regulating the ratio of peripheral blood T lymphocyte subsets and reducing proinflammatory cytokine release . Methods Twelve-week SHR and WKY rats were randomly divided into three groups :WKY group ,SHR group and SHR+NO intervention group ,with 6 rats in each group .Rats in SHR+ NO intervention group were injected intraperitoneally with sodium nitroprusside according to 10 μg/(kg · d) for 4 weeks ,while WKY group and SHR group were injected intraperitoneally with an equal amount of saline for 4 weeks .After measuring the tail artery blood pressure ,basilar artery and renal pathological changes were observed by HE staining ;the rates of CD4+ /CD8+ and CD4+ CD25+ T cells were detected by flow cytometry ;and the expression of TNF-α was detected by ELISA .Results The expressions of CD4+ /CD8+ and TNF-αin SHR group were significantly higher than those in WKY group (P<0 .01) while the rate of CD4+ CD25+ in SHR group was significantly lower than that in WKY group (P<0 .01) .The expressions of CD4+ /CD8+ and TNF-α in SHR+ NO intervention group were significantly lower than those in SHR group whereas the rate of CD4+ CD25+ was significantly higher than that in SHR group (P<0 .05) .Conclusion NO can improve the target organ damage caused by hypertension by regulating the peripheral blood T lymphocyte subsets ratio and reducing the release of proinflammatory factors .
