Correlation of Liver Depression Grading with Estrogen Receptor Alpha and Beta,G Protein-coupled Receptor 30 of Perimenopausal Women with Non-organic Insomnia
10.13359/j.cnki.gzxbtcm.2018.01.002
- VernacularTitle:围绝经期非器质性失眠妇女肝郁分级与雌激素 受体α、β,GPR30的相关性研究
- Author:
Hong LI
1
;
Ying CHEN
;
Jiao Bi GONG
;
Kun MA
;
Guang Shao LYU
;
Qin Jiang ZHENG
Author Information
1. 福建医科大学省立临床医学院
- Keywords:
perimenopause;
liver depression;
non-organic insomnia;
estrogen receptors
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2018;35(1):6-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation of liver depression grading with estrogen receptor alpha and beta(ERα,ERβ)and G protein-couple receptor 30(GPR30)of the perimenopausal women suffering from non-organic insomnia. Methods A total of 127 perimenopausal women suffering from non-organic insomnia were enrolled into the study. The data collected by four diagnostic methods and the scores of Pittsburgh Sleep Quality Index(PSQI)were used for the scoring and grading of liver depression according to Syndrome Element Differentiation. The mRNA and protein expression levels of ERα,ERβand GPR30 in the mononuclear cells of peripheral blood from the patients were detected with real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and Western blot (WB)method respectively. Results (1)The differences of mRNA levels of ERα,ERβ and GPR30 were insignificant among the patients with different degrees of liver depression (P > 0.05). ERα mRNA/ERβ mRNA ratio was decreased with the increase of liver depression grading,the differences being significant (F = 6.710, P < 0.001). (2) The differences of protein levels of ERα, ERβ and GPR30 were insignificant among the patients with different degrees of liver depression (P > 0.05);ERα/ERβ protein gray value ratio was decreased with the increase of liver depression grading, and the differences were significant (P < 0.05) . Conclusion The decline of ERα/ERβ is probably contributed to the pathological basis of liver depression in perimenopausal non-organic insomnia women.
