Epstein-Barr virus and gastrointestinal lymphomas in Korea.
10.3349/ymj.1998.39.3.268
- Author:
Woo Ick YANG
1
;
Min Sun CHO
;
Yasuhiko TOMITA
;
Masahiko OHSAWA
;
Katsuyuki AOZASA
Author Information
1. Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Epstein-Barr virus;
polymerase chain reaction;
in situ hybridization;
gastrointestinal lymphomas
- MeSH:
Gastrointestinal Neoplasms/virology*;
Gastrointestinal Neoplasms/pathology;
Genome, Viral;
Herpesvirus 4, Human/isolation & purification*;
Herpesvirus 4, Human/genetics;
Human;
In Situ Hybridization;
Korea;
Lymphoma/virology*;
Lymphoma/pathology;
Polymerase Chain Reaction
- From:Yonsei Medical Journal
1998;39(3):268-276
- CountryRepublic of Korea
- Language:English
-
Abstract:
To analyze the association of Epstein-Barr virus (EBV) with gastrointestinal non-Hodgkin's lymphomas arising in immunocompetent patients, 56 consecutive cases of gastrointestinal lymphomas (B-cell: 52-cases, T-cell: 3 cases, T/NK-cell: 1 case) occurring in the stomach (33 cases), intestine (22 cases) and esophagus (1 case) were investigated for the presence of EBV using polymerase chain reaction analysis as a screening method followed by EBER-1 RNA and DNA in situ hybridization (ISH) and immunohistochemistry for the expression of latent membrane protein 1 (LMP-1). Forty-seven cases demonstrated extractable DNA and EBV DNA was detected only in 4 cases. Among the, RNA (EBER-1) and DNA ISH analysis confirmed the presence of the EBV genome in tumor cells in 3 cases (T/NK-cell lymphoma of ileum, gastric high-grade B-cell lymphoma of mucosa-associated lymphoid tissue, gastric diffuse large B-cell lymphoma). Only the T/NK cell lymphoma showed diffuse positivity of tumor cells while 2 gastric B-cell lymphomas demonstrated a scattered positive reaction and no cases expressed LMP-1. Nine cases without extractable DNA by the PCR method showed no nuclear signal by EBER-1 ISH. These findings suggest that most sporadic primary gastrointestinal lymphomas in Korea are not associated with EBV.
