Cryptosporidium infection in Nanjing City from 2015 to 2016
10.16250/j.32.1374.2017134
- VernacularTitle:2015-2016年南京市隐孢子虫抽样调查报告
- Author:
cai Pei YANG
1
;
ying Hong ZHANG
;
Wei ZHOU
;
gang Wei YIN
;
Pei WU
;
Ke ZHANG
;
sheng Hong FU
;
rong Ben YOU
;
xin Li WAN
;
Jun YOU
Author Information
1. 南京市疾病预防控制中心 南京210003
- Keywords:
Sampling survey;
Gold amine phenol-modified acid-fast staining;
Fluorescence quantitative PCR;
Cryptosporid-ium;
Nanjing City
- From:
Chinese Journal of Schistosomiasis Control
2017;29(6):752-755
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the status of Cryptosporidium infection in the population in Nanjing City so as to pro-vide the evidence for the prevention and control of cryptosporidiosis. Methods A total of 100 fecal samples were collected from each of three districts(Liuhe,Qixia and Gaochun)and one hospital(Nanjing Zhongda Hospital)in 2015 and 2016 respective-ly. The fecal samples were detected for Cryptosporidium with microscopy(by using the gold amine phenol-modified acid-fast staining)and the positive samples were detected again for the molecular biology confirming by using the fluorescence quantita-tive PCR. Results During the two years,581 cases of normal population who lived in the city were surveyed and no Cryptospo-ridium infection was found. Among 202 cases of outpatients with chronic diarrhea,there were 9 Cryptosporidium positive cases with the microscope scanning method (4.46％),and among the 9 cases,7 cases showed obvious logarithmic amplification curves showing positive Cryptosporidium nucleic acid,but 2 cases without the obvious logarithmic amplification curves,and the Cryptosporidium nucleic acid positive rate was 3.47％. Conclusions Cryptosporidium infection is not found in the normal popu-lation of Nanjing City,but the Cryptosporidium infection is found in the chronic diarrhea patients. The results imply that we should strengthen the detection of Cryptosporidium in the chronic diarrhea patients,so as to provide the evidence for improving the diagnosis and treatment of cryptosporidiosis.
