Preliminary research on prokaryotic expression and immune protection of triosephosphate isomerase of Toxoplasma gondii
10.16250/j.32.1374.2017157
- VernacularTitle:弓形虫磷酸丙糖异构酶原核表达及免疫保护的初步研究
- Author:
Shuang SHEN
1
,
2
,
3
;
上海交通大学附属第六人民医院
;
江南大学公共卫生研究中心
;
ren Xu YIN
;
jun Li SONG
;
Jie WANG
;
dan Xue KE
;
Wei ZHOU
;
xin Chuan YU
Author Information
1. 上海健康医学院附属第六人民医院东院 上海201306
2. 上海交通大学附属第六人民医院
3. 江南大学公共卫生研究中心
- Keywords:
Toxoplasma gondii;
Triosephosphate isomerase;
Prokaryotic expression;
Immune protection
- From:
Chinese Journal of Schistosomiasis Control
2017;29(6):780-783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the prokaryotic expression and immune protection of triosephosphate isomerase(TPI)of Toxoplasma gondii in mice. Methods Total RNA was extracted from toxoplasma tachyzoites,and TPI fragment was amplified by PCR and cloned into the prokaryotic expression vector pET-28a(+). The target protein was induced with IPTG and purified by Ni-NTA affinity chromatography. The mice were immunized 4 times by emulsified TPI with adjuvant,and the last time was the strengthen immunization. At the same time,an adjuvant group and a normal group were set as controls. The blood samples were got from the tail vein of the mice,and the serum antibody titres were detected. All the mice were challenged with 400 toxo-plasma tachyzoites to observe the survival time. Results The TPI gene was amplified from T. gondii cDNA by PCR. The recom-binant vector TPI/pET-28a(+)was usefully constructed,and the TPI protein was expressed and purified. The serum antibody ti-tre could be more than 100 thousand. After infected with toxoplasma tachyzoites,the survival time of the mice in the experimen-tal group was longer than that of the mice in the control groups. Conclusion The TPI protein of T. gondii could trigger the im-munoprotection against T. gondii challenge in the mice.
