Study on the toxicokinetics of cymermethrin and its metabolites in dog bile
10.13618/j.issn.1001-5728.2017.06.003
- VernacularTitle:氯氰菊酯及其代谢物在犬胆汁中的代谢动力学研究
- Author:
Feng ZHENG
1
;
Yanan SHEN
;
Aiai FAN
;
Juan JIA
;
Zhiwen WEI
;
Yao LIU
;
Bin CONG
;
Keming YUN
Author Information
1. 山西医科大学法医学院
- Keywords:
forensic toxicology;
HPLC-MS-MS;
bile;
cymermethrin;
metabolites
- From:
Chinese Journal of Forensic Medicine
2017;32(6):557-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the toxicokinetics of Cymermethrin and its metabolites in dog bile and provide evidence for forensic cases of identification of Cymermethrin poisoning. Methods 1/4LD50 doses of Cymermethrin were given to 6 male dogs by oral perfusion after the gallbladder fistula surgery on them,and their bile were collected at different time, in which Cymermethrin and its metabolites were extracted by Liquid-liquid extraction with dichloromethane and detected by HPLC-MS-MS. The qualitative analysis was based on retention time and MRM ions. The quantitative analysis was based on an internal standard method and calibration curve. Toxicokinetics equations of Cymermethrin and its metabolites in the bile were established from the c-t curves which were fitted by the WinNonlin toxicokinetics software meanwhie toxicokinetics parameters were obtained. Results The toxicokinetics of Cymermethrin met first-order dynamic equation. The Tmax of Cymermethrin(CYM), 3-phenoxybenzoic acid (3-PBA), 3-(2,2-Dichloroethenyl)-2,2-dimethyl-cyclopropanecarboxylate (DCVA) respectively were 1.52±0.30,1.29±0.04,0.93±0.41 h ; The Cmax of CYM, 3-PBA, DCVA respectively were 0.38±0.03,7.9±1.32,30.9±16.24 μg/mL ; The T1/2 of CYM, 3-PBA, DCVA respectively were3.93±0.71,1.36±0.11,4.49±2.81 h; Conclusion The toxicokinetics of Cymermethrin in dog bile met first-order dynamic equation ; The toxicokinetics model and parameters of Cymermethrin can provide evidence for forensic identification of Cymermethrin poisoning cases.
