Effects and the mechanism of triptolide on proliferation and apoptosis of breast cancer MCF-7 cells
10.3969/j.issn.1006-5725.2017.23.005
- VernacularTitle:雷公藤甲素对乳腺癌MCF-7细胞增殖和凋亡的影响及其机制
- Author:
Maojian CHEN
1
;
Chanchan XIAO
;
Li WANG
;
Weiping YANG
;
Qinghong QIN
;
Changyuan WEI
Author Information
1. 广西医科大学附属肿瘤医院乳腺外科
- Keywords:
Triptolide;
Breast cancer;
Apoptosis;
Bcl-2;
Bax;
Survivin;
Caspase-3
- From:
The Journal of Practical Medicine
2017;33(23):3867-3870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects and mechanism of triptolide on proliferation and apoptosis of breast cancer MCF-7 cells.Methods MCF-7 cells were treated by different concentrations of triptolide.CCK-8 as-say was employed to detect the cell proliferation. The morphological changes were observed by an inverted micro-scope.The apoptosis rate was detected by flow cytometry.Expressions of Bcl-2,Bax,Survivin and Caspase-3 were measured by qRT-PCR and Western blot. Results Triptolide inhibited the proliferation of MCF-7 cells in a dose and time-dependent manner at a suitable range.Triptolide induced morphological changes and apoptosis.Triptolide also down-regulated Bcl-2 and Survivin expressions and up-regulated Bax and Caspase-3 expressions. Conclu-sions Triptolide inhibits proliferation and induces apoptosis of MCF-7 cells,and its mechanism may be related to down-regulation of Bcl-2 and Survivin expressions and up-regulation of Bax and Caspase-3 expressions.
