Role of combination of autophagy inhibition and anti-VEGF in angiogenesis of retinal vascular endothelial cells
10.3969/j.issn.1006-5725.2017.24.007
- VernacularTitle:自噬抑制联合抗-VEGF对视网膜血管内皮细胞血管生成的作用
- Author:
Meng CHEN
1
;
Rong LI
;
Yang YAO
;
Tianzhi CAI
;
Zhaoxiang YU
;
Xiaobo LIN
Author Information
1. 西安交通大学附属三二〇一医院眼科
- Keywords:
angiogenesis;
retinal neovascularization;
autophagy;
VEGF;
treatment
- From:
The Journal of Practical Medicine
2017;33(24):4048-4052
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,we explored the role of combination of autophagy inhibition and anti-VEGF in proliferation,migration and tube formation of mouse retinal vascular endothelial cells(RVECs). Methods Well cultured mouse RVECs were randomly divided into four groups:autophagy inhibition group(add-ing autophagy inhibitor 3-MA),anti-VEGF group(adding anti-VEGF-A neutralized antibody),autophagy inhibi-tion+anti-VEGF group(adding the two reagents)and the control group.All cells were then cultured in the hypoxic condition. The cell proliferation,migration and tube formation were detected by EdU,transwell and matrigel as-say,respectively. Results The cell proliferation rate,number of migrated cells and number of tube formation of the other three groups decreased when compared with the control group.These data above in autophagy inhibition+anti-VEGF group were all significantly less than 3-MA group and anti-VEGF group. Conclusion Combination of autophagy inhibition and anti-VEGF may be more effective than simple anti-VEGF in inhibition of retinal neovascu-larization.
