Expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions
10.3969/j.issn.1006-5725.2017.24.021
- VernacularTitle:DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义
- Author:
Jiayu CAO
1
,
2
;
华北理工大学附属医院妇产科
;
Jie BAI
;
Jianxin DONG
;
Ting SHAO
;
Yan CHEN
Author Information
1. 063000 河北省唐山市,华北理工大学基础医学院、河北省慢性疾病重点实验室、唐山市慢性病临床基础研究重点实验室
2. 华北理工大学附属医院妇产科
- Keywords:
DNA methyltransferase 1;
Cyclin A1;
cervical cancer;
cervical precancerous lesions
- From:
The Journal of Practical Medicine
2017;33(24):4105-4109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions. Methods Cervical tissues were selected from normal cervical(NC),low-grade cervical intraepithelial lesion(LSIL),high-grade cervical intraepithelial lesion(HSIL)and cervical squa-mous cell carcinoma(SCC)from each thirty patients.The expression of DNMT1 and CCNA1 mRNA and protein was examined by Western Blot analysis and qRT-PCR in cervical tissues. Results The expression of DNMT1 mRNA and protein in LSIL,HSIL and SCC was higher than in NC(F=117.93,P<0.05;F=61.24,P<0.05). Expression of DNMT1 mRNA and protein was increased steadily according to severity of cervical lesions(χ2trend=26.25,P<0.05;χ2trend=26.60,P<0.05).The expression of CCNA1 mRNA and protein in HSIL and SCC was lower than that in NC and LSIL(F = 77.04,P < 0.05;F = 57.15,P < 0.05). Expression of CCNA1 mRNA and protein was decreased steadily according to severity of cervical lesions(χ2trend=64.19,P<0.05;χ2trend=60.24,P<0.05). There was a negative correlation between expression of DNMT1 and CCNA1 protein in LSIL,HSIL,SCC (r=-0.75,-0.56,P<0.05). Conclusion In DNMT1 mRNA there is high expression of protein and in CCNA1 mRNA there is low expression of protein,but both may be related to the occurrence and development of cervical cancer.
