Preparation of recombinant retrovirus pRevTRE-E77.43 and its protective effect in a mouse model of Schistosoma japonicum infection
10.16250/j.32.1374.2017164
- VernacularTitle:重组逆病毒pRevTRE-E77.43转导小鼠后抗血吸虫感染作用
- Author:
sheng Fan ZENG
1
;
qun Sai LUO
;
hui De XIONG
;
jing Yuan YU
;
jun Ying QIAN
;
qiang Zhi QIN
Author Information
1. 益阳市洞庭湖区血吸虫与病原生物控制技术重点实验室
- Keywords:
Schistosoma japonicum;
Microtus fortis;
Recombinant retrovirus;
Resistance associated gene;
Effect of worm re-duction
- From:
Chinese Journal of Schistosomiasis Control
2017;29(6):730-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological functions of E77.43, a gene segment of Microtus fortis, in treating Schistoso-ma japonicum infection. Methods Recombinant retroviral vectors of pRevTRE-E77.43 was constructed, and recombinant retro-viral vectors were transfected into PA317 cells, and the stable cell lines were obtained by hygromycin screening, followed by the packaging, concentration and purification of recombinant retrovirus. The virus was transferred to the mice infected by S. japoni-cum via intravenous or intraperitoneal injection, through which the express of target gene and the treatment function in vivo were observed. Results The experiment showed the recombinant virus injected mice could efficiently express E77.43 on the 7th day after the injection which lasted for forty-five days thereafter. A significant reduction in adult worms (31.0％) and a high reduction (35.0％) in liver eggs were induced by pRevTRE-E77.43, while the reduction in adult worms and that in liver eggs was 1.2％and 0.9％induced by pRevTRE respectively (t=3.524, 9.485, both P<0.01). Conclusion pRevTRE-E77.43 could be used for the treatment of S. japonicum infection, indicating that E77.43 may involve in the natural resistance of M. fortis to S. japonicum infec-tion.
